comparison between orbital fat-derived stem cells and bone marrow-derived mesenchymal and adipose-derived stem cells in human
Authors
abstract
purpose: to separate and characterize orbital fat-derived stem cells (ofscs) and compare its cellular and molecular properties with those of bone marrow-derived mesenchymal stem cells (mscs) and adipose-derived stem cells (ascs). methods: stem cells from orbital fat, abdominal fat and bone marrow aspirates have been isolated. we assessed stem cell specific cell surface markers using cytometry, osteoblast and adipocyte differentiation potential using special stains, and cellular proliferation rate using mtt test and expression of pleuripotency genes by real-time polymerase chain reaction (pcr). we also assayed wound healing using a scratch test and expression of corneal epithelial specific cell markers using an air lifting test. results: results of flow cytometry showed that ofscs were positive for cd105, cd90, cd44, cd166 and cd73 and negative for cd34, cd45, cd31 and cd133, similar to mscs and ascs. differentiation to osteoblast and adipocyte was observed in all groups. ofscs healed more rapidly as compared to mscs and ascs. only ofscs expressed epithelial markers krt3, krt12 and connexin43 after air lifting. conclusion: orbital fat-derived stem cells had a high capability of proliferation and differentiation. given the possibility of expression of epithelial markers and the potential of regeneration as well as its close location to the eye, these cells can be considered for cell therapy to treat persistent corneal epithelial defects.
similar resources
Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells
Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from a variety of adult and neonatal tissues including the adipose tissue. Stemness and multipotential features of Mesenchymal Stem Cells (MSC) has been highlighted in many studies but there are many dark aspects in ex...
full textBiological behaviors of muscarinic receptors in mesenchymal stem cells derived from human placenta and bone marrow
Objective(s): Cells perform their functional activities by communicating with each other through endogenous substances and receptors. Post-translation, stem cells function properly in new host tissue by carrying specific cell surface receptors. We aimed to characterize muscarinic receptor subtypes in mesenchymal stem cells (MSCs) together with osteogenic and adipogenic...
full textComparison of Characteristics of Human Amniotic Membrane and Human Adipose Tissue Derived Mesenchymal Stem Cells
BACKGROUND Mesenchymal stem cells (MSCs) are ideal candidates for treatment of diseases. Amniotic membranes are an inexpensive source of MSCs (AM-MSC) without any donor site morbidity in cell therapy. Adipose tissue derived stem cells (ASCs) are also suitable cells for cell therapy. There is discrepancy in CD271 expression among MSCs from different sources. In this study, the characteristics...
full textCytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells
full text
Differentiation of Adipose-derived Stem Cells into Schwann Cell Phenotype in Comparison with Bone Marrow Stem Cells
Objective(s) Bone marrow is the traditional source of human multipotent mesenchymal stem cells (MSCs), but adipose tissue appears to be an alternative and more readily available source. In this study, rat adipose-derived stem cells (ADSCs) were induced to differentiate into Schwann-like cells and compared with rat bone marrow stem cells (BMSCs) for their Schwann-like cells differentiation pote...
full textMatrigel Enhances in vitro Bone Differentiation of Human Marrow-derived Mesenchymal Stem Cells
Objective(s) The use of co-culture cells as well as extra cellular matrix are among those strategies that have been employed to direct mesenchymal stem cell (MSC) bone differentiation in culture. In this regard, there is no study considering the effects of Matrigel on mesenchymal stem cell (MSC) in vitro bone differentiation. This was the subject of the present study. Materials and Methods ...
full textMy Resources
Save resource for easier access later
Journal title:
بیناجلد ۲۱، شماره ۱، صفحات ۱۶-۲۶
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023